Methodology


Genotyping
DNA preparation
For PCR, DNA can be purified using commercial kits or alternatively thermolysates are prepared by heating bacteria in TE for 20min at 95C then cooling in ice and centrifugation. The supernatant is kept frozen at -20C.

PCR-based methods
DR analysis
MLVA 16
RD 105
Other techniques
IS110 RFLP